MLPA® is the gold standard for DNA copy number quantification and is used to study both hereditary disorders and tumours. MLPA can also be applied to investigate the methylation status of DNA sequences. MRC-Holland, the inventor and manufacturer of MLPA, offers 400 different MLPA panels. Popular applications include:
Predisposition to Cancer
BRCA1, BRCA2, MLH1,MSH2, MSH6, PMS2, APC, MMR, NF1
Neuromuscular Disorders
Intellectual Disability
microdeletion, PWS/AS, FMR1/AFF2, MECP2, Sotos, WBS, SHOX, aneuploidy
Solid Tumours
Gliomas, MMR, Neuroblastoma, TP53, Breast
Methylation profiling
Prader-Willi / Angelman syndrome, Fragile X, Beckwith-Wiedemann Syndrome
Advantages and features of MLPA
- Examine up to 60 targets per reaction
- Only 50ng of DNA is required per reaction
- MLPA detects a short sequence of 60-80 nucleotides
- Detection of single exon deletions and duplications
- Detection of targeted point mutations
- One simple protocol for >400 applications
- Results available within 24 hours
- Limited lab equipment required: only a thermocycler and a sequence type electrophoresis system
- User-friendly, reliable, and free data analysis software, Coffalser.NetTM